Education:
1986 B.S. in Biology,
University of Science and Technology of China, Hefei, China.
1994 Ph.D. in Biophysics,
University of Illinois at Urbana-Champaign, Urbana, IL.
Professional
Experience:
9/86-5/89
Graduate research assistant, Institute of Biophysics, Chinese
Academy of Sciences, Beijing, China. (Advisor: Prof. Dong-Cai Liang)
9/89-8/94
Graduate research assistant, Biophysics Division, University of
Illinois at Urbana-Champaign, Urbana, IL. (Advisor: Prof. Andrew H.-J.
Wang)
9/94-10/98
Howard Hughes Medical Institute postdoctoral research associate,
University of Texas Southwestern Medical Center at Dallas. (Advisor: Prof.
Johann Deisenhofer)
11/98-8/99
Research associate, Center for Advanced Research in Biotechnology,
University of Maryland, Rockville, MD. (Advisor: Prof. Osnat Herzberg)
9/99-2/2005 Assistant
Professor, Department of Biochemistry, UT Southwestern Medical Center at
Dallas, TX.
2/2005-present
Associate Professor, Department of Biochemistry, UT Southwestern Medical
Center at
Dallas, TX.
Publications:
31. Karthikeyan, S., Zhou, Q., Zhao, Z., Kao, C.-L., Tao, Z., Robinson,
H., Liu H.-w. and Zhang, H. (2004).
Structural analysis of Pseudomonas
1-aminocyclopropane-1-carboxylate deaminase complexes: Insight into the
mechanism of a unique pyridoxal-5’-phosphate dependent cyclopropane
ring-opening reaction.. Biochemistry,
43, 13328-13339.
30. Ginalski, K, Zhang,
H. and Grishin, N. V. (2004). Raptor
protein contains a caspase-like domain. Trends in Biomed. Sci,
29, 522-524.
29. Karthikeyan, S., Zhao, Z., Kao, C., Zhou, Q., Tao, Z.,
Zhang, H. and Liu, H.-w. (2004).
Structural Analysis of 1-Aminocyclopropane-1-carboxylate Deaminase:
Observation of an Aminyl Intermediate and Identification of Tyr294 as the
Active-site Nucleophile. Angew.
Chem Int. Ed.. 43, 3425-3429.
28.
Zhang, H. (2004). Protein prenyltransferases. In Handbook of
Metalloproteins, Volume 3. p37-48.
A. Messerschmidt, W.
Bode, and M.Cygler eds. (John Wiley & Sons). Invited book
chapter.
27. Singh, S.K., Yang, K., Karthikeyan, S., Huynh, T., Zhang, X.,
Phillips, M. A., Zhang, H. (2004). The thrH gene product of Pseudomonas
aeruginosa is a dual activity enzyme with a novel
phosphoserine:homoserine phosphotransferase activity. J. Biol.
Chem. 279, 13166-13173.
26. Karthikeyan, S.,
Zhou, Q., Osterman, A. L. and Zhang, H. (2003) Ligand-binding
induced conformational changes in riboflavin kinase: structural basis for
the ordered mechanism. Biochemistry, 42,
12532-12538.
25. Zhang, H. (2003).
Binding platforms for Rab prenylation and recycling: Rab escort protein,
RabGGT and RabGDI.
Structure 11, 237-239.
24. Zhang, X.,
Kurnasov, O. V., Karthikeyan, S., Grishin, N.V., Osterman, A. L. and
Zhang, H. (2003). Structural characterization of a human
cytosolic NMN/NaMN adenylyltransferase and implication in human NAD
biosynthesis. J. Biol. Chem. 278,
13503-13511.
23.
Karthikeyan, S., Zhou, Q., Mseeh, F., Grishin, N.
V., Osterman, A. L. and Zhang, H. (2003). Crystal structure of
human riboflavin kinase reveals a b barrel fold and a
novel active site arch. Structure
11,
265-273.
22.
Lim, K., Zhang, H., Toedt, J., Tempcyzk, A., Krajewski, W., Howard,
A., Eisenstein, E., Herzberg, O. (2003). Structure of YibK
methyltransferase from Haemophilus influenzae (HI0766): a cofactor
bound at a site formed by a knot.
Proteins
Struc. Func. and Genetics,
51, 56-67.
21. Gilliland GL, Teplyakov A,
Obmolova G, Tordova M, Thanki N, Ladner J, Herzberg O, Lim K, Zhang H,
Huang K, Li Z, Tempczyk A, Krajewski W, Parsons L, Yeh DC, Orban J, Howard
AJ, Eisenstein E, F Parsons J, Bonander N, Fisher KE, Toedt J, Reddy P,
Rao CV, Melamud E, Moult J. (2002). Assisting functional assignment
for hypothetical Heamophilus influenzae gene products through
structural genomics. Curr
Drug Targets Infect Disord. 2, 339-953.
20. Chen, C.
C., Zhang, H., Kim, A. D., Howard, A., Sheldrick, G. M.,
Mariano-Dunaway, D., Herzberg, O. (2002). Degradation Pathway of
the Phosphonate Ciliatine: Crystal Structure of 2-Aminoethylphosphonate
Transaminase. Biochemistry
41,13162-13169.
19. Singh, S. K.,
Kurnasov, O. V., Chen, B., Robinson, H., Grishin, N. V., Osterman, A. L.
and Zhang, H. (2002). Crystal structure of Haemophilus
influenzae NadR protein: a bifunctional enzyme endowed with NMN
adenylyltransferase and ribosylnicotinamide kinase activities. J.
Biol. Chem., 277, 33291-33299.
18.
Cheek, S., Zhang, H. and Grishin, N. V. (2002). Sequence and
Structure Classification of kinases. J. Mol. Biol.
320, 855-881.
17.
Zhou, T., Kurnasov, O. V., Tomchick, D., Binns, D., Grishin, N. V.,
Marquez, V. E., Osterman, A. L. and Zhang, H.. (2002).
Structure of human nicotinamide/nicotinic acid mononucleotide
adenylyltransferase: basis for the dual substrate specificity and
activation of anticancer drug tiazofurin. J. Biol. Chem.,
277, 13148-13154.
16. Zhang, H., Zhou, T., Kurnasov, O. V., Cheek, S., Grishin,
N. V. and Osterman, A. L. (2002). Crystal structure of E. coli
nicotinate mononucleotide adenylyltransferase and its complex with
deamido-NAD. Structure, 10, 69-79.
15. Lim, K., Zhang, H., Tempczyk, A., Bonander, N., Toedt,
J., Howard, A., Eisenstein, E. and Herzberg, O. (2001). Crystal Structure
of YecO from Haemophilus influenzae (HI0319) reveals a
methyltransferase fold and a bound S-adenosylhomocysteine. Proteins
Struc. Func. and Genetics,
45,
397-407.
14. Sri Krishna, S., Zhou, T., Daugherty, M., Osterman A. L. and
Zhang, H. (2001). Structural basis for the catalysis and substrate
specificity of homoserine kinase. Biochemistry, 40,
10810-10818.
13.
Zhou, T.,
Daugherty, M., Grishin, N. V., Osterman, A. L. and Zhang, H.
(2000). Structure and Mechanism of Homoserine Kinase: prototype for GHMP
kinase superfamily. Structure.
8, 1247-1257
(Works
from graduate school and postdoc years)
12. Zhang, H., Huang, K., Li, Zhong, Banerjee, L., Fisher,
K., Eisenstein, E., Grishin, N. V. and Herzberg, O. (2000). Crystal
structure of YbaK protein from Haemophilus influenzae at 1.8Å
resolution: structure-functional implications. Proteins Struc. Func.
and Genetics. 40, 86-97.
11. Zhang, H., Seabra, M. and Deisenhofer, J. (2000). Crystal
structure of Rab geranylgeranyl transferase at 2.0Å resolution. Structure. 8, 241-251.
10. Zhang, H. and Grishin N. V. (1999). The a
subunt of protein prenyltransferases is a member of the tetratricopeptide
repeat family. Protein
Science. 8, 1658-1667.
9. Sevrioukova, I. F., Li, H., Zhang, H., Peterson, J. A. and
Poulos, T. L. (1999). Structure of a cytochrome P450-redox partner
electron-transfer complex. Proc.
Natl. Acad. Sci. USA.
96, 1863-1868.
8.
Su, S., Gao, Y.G., Zhang, H., Terwilliger, T.C. and Wang, A.H.-J.
(1997). Analysis of the stability and function of three surface mutants
(R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray
crystallography. Protein
Science. 6:771-80
7. Zhang, H., Skinner, M. M., Sandberg, W. S., Wang, A. H.-J.
and Terwilliger, T. C. (1996). Context-dependence of mutational effects in
a protein: the crystal structures of the V35I, I47V and V35I/I47V gene V
protein core mutants. J. Mol.
Biol. 259, 148-159.
6. Guan, Y., Zhang, H. and Wang, A. H.-J. (1995).
Electrostatic potential distribution of the gene V protein from Ff phage
facilitates cooperative DNA binding: a model of the GVP-ssDNA complex.
Protein Science. 4, 187-197.
5.
Guan, Y., Zhang, H., Konings, R. N. H., Hilbers, C. W.,
Terwilliger, T. C. & Wang, A. H.-J. (1994). Crystal structures of Y41H
and Y41F mutants of gene V protein from Ff phage suggest possible
protein-protein interactions in GVP-ssDNA complex. Biochemistry. 33,
7768-7778.
4. Skinner, M. M., Zhang, H., Leschnitzer, D. H., Guan, Y.,
Bellamy, H., Sweet, R. M., Gray, C. W., Konings, R. N. H., Wang, A. H.-J.
and Terwilliger, T. C. (1994). Structure of the gene V protein of
bacteriophage f1 determined by multiwavelength X-ray diffraction on the
selenomethionyl protein. Proc.
Natl. Acad. Sci. USA.
91, 2071-2075.
3. Zhang, H., Gao, Y.-G., van der Marel, G. A., van Boom, J.
H. and Wang, A. H.-J. (1993). Simultaneous incorporation of two anticancer
drugs into DNA: the structures of cross-linked adducts of
Daunorubicin-d(CG(araC)GCG) and Doxrubicin-d(CA(araC)GTG) complexes at
high resolution. J. Biol. Chem. 268, 10095-10101.
2. Wang, H.-J. A., Sriram, M., Gao, Y.- G., Robinson, H., Jean, Y.-
C., Li, Y.- K. and Zhang, H. (1992). Anticancer drug daunorubicin
and its derivatives can be crosslinked to DNA efficiently by formaldehyde:
possible clues for drug design.
Structure &
Function, Volume 1:
Nucleic Acids. Eds. Sarma & Sarma, Adenine Press.
1. Zhang, H., van der Marel, G. A., van Boom, J. H. and Wang,
A. H.-J. (1992). Conformational perturbation of the anticancer nucleoside
arabinosylcytosine on Z-DNA: molecular structure of (araC-dG)3
at 1.3 Å resolution. Biopolymers. 32, 1559-1569.
|
